> Restriction Endonucleases > Xho I

Xho I


 
 
 Recognition site:
 
 CTCGAG
GAGCTC
 
 Activity, Fermentas:
 
Blue+ Green+ Orange+ Red+ Yellow+ 1x Yellow+ 2x
0-20% 50-100% 50-100% 100% 20-50% 100%
 
 
 Activity, NEB:
 
NEBuffer 1 NEBuffer 2 NEBuffer 3 NEBuffer 4
75% 100% 100% 100%
 
 Activity, Promega:
 
 
A B C D MULTI-CORE
2550% 75100% 75100% 100% 1025%
 
 Recommended buffer: 
Fermentas:Buffer R+
NEB:NEBuffer 2 + BSA 50 mM
Promega:Buffer D
 
 Incubation temperature:  37C
 
 Heat Inactivation:  65C for 20 minutes.
 
 Links:
 
  Xho I, Fermentas
Xho I, NEB
Promega
 
 



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