> Restriction Endonucleases > Sph I

Sph I


 
 
 Recognition site:
 
 GCATGC
CGTACG
 
 
 Activity, NEB:
 
NEBuffer 1 NEBuffer 2 NEBuffer 3 NEBuffer 4
100% 100% 50% 100%
 
 Activity, Promega:
 
 
A B C D K MULTI-CORE
75100% 75100% 100% 75100% 100% 1025%
 
 Recommended buffer: 
NEB:NEBuffer 2
Promega:Buffer K
 
 Incubation temperature:  37C
 
 Heat Inactivation:  65C for 20 minutes.
 
 Links:
 
 
Sph I, NEB
Promega
 
 



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