> Restriction Endonucleases > Sma I

Sma I


 
 
 Recognition site:
 
 CCCGGG
GGGCCC
 
 Activity, Fermentas:
 
Blue+ Green+ Orange+ Red+ Yellow+ 1x Yellow+ 2x
50-100% 0-20% 0-20% 0-20% 100% 0-20%
 
 
 Activity, NEB:
 
NEBuffer 1 NEBuffer 2 NEBuffer 3 NEBuffer 4
0% 0% 0% 100%
 
 Activity, Promega:
 
 
A B C D J MULTI-CORE
<10% <10% <10% <10% 100% 100%
 
 Recommended buffer: 
Fermentas:Y+/Tango
NEB:NEBuffer 4
Promega:Buffer J
 
 Incubation temperature:  30C
 
 Heat Inactivation:  65C for 20 minutes.
 
 Links:
 
  Sma I, Fermentas
Sma I, NEB
Promega
 
 



"Practical Molecular Biology" http://molbiol.edu.ru
e-mail: pmb@molbiol.edu.ru


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