> Restriction Endonucleases > Sca I

Sca I


 
 
 Recognition site:
 
 AGTACT
TCATGA
 
 Activity, Fermentas:
 
Blue+ Green+ Orange+ Red+ Yellow+ 1x Yellow+ 2x
0-20% 0-20% 0-20% 0-20% 0-20% 0-20%
 
 
 Activity, NEB:
 
NEBuffer 1 NEBuffer 2 NEBuffer 3 NEBuffer 4
25% 75% 50% 25%
 
 Activity, Promega:
 
 
A B C D K MULTI-CORE
<10% 100% 5075% 75100% 100% 1025%2
 
 Recommended buffer: 
Fermentas:Buffer Sca I+
NEB:NEBuffer Sca
Promega:Buffer K
 
 Incubation temperature:  37C
 
 Heat Inactivation:  80C for 20 minutes.
 
 Links:
 
  Sca I, Fermentas
Sca I, NEB
Promega
 
 



"Practical Molecular Biology" http://molbiol.edu.ru
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