> Restriction Endonucleases > Not I

Not I


 
 
 Recognition site:
 
 GCGGCCGC
CGCCGGCG
 
 Activity, Fermentas:
 
Blue+ Green+ Orange+ Red+ Yellow+ 1x Yellow+ 2x
0-20% 0-20% 100% 20-50% 0-20% 20-50%
 
 
 Activity, NEB:
 
NEBuffer 1 NEBuffer 2 NEBuffer 3 NEBuffer 4
0% 75% 100% 50%
 
 Activity, Promega:
 
 
A B C D MULTI-CORE
<10% 1025% 2550% 100% 2550%
 
 Recommended buffer: 
Fermentas:Buffer O+
NEB:NEBuffer 3 + BSA 100 mM
Promega:Buffer D
 
 Incubation temperature:  37C
 
 Heat Inactivation:  65C for 20 minutes.
 
 Links:
 
  Not I, Fermentas
Not I, NEB
Promega
 
 



"Practical Molecular Biology" http://molbiol.edu.ru
e-mail: pmb@molbiol.edu.ru


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