> Restriction Endonucleases > Mnl I

Mnl I


 
 
 Recognition site:
 
 CCTC(N)7
GGAG(N)6
 
 Activity, Fermentas:
 
Blue+ Green+ Orange+ Red+ Yellow+ 1x Yellow+ 2x
50-100% 100% 20-50% 20-50% 20-50% 20-50%
 
 
 Activity, NEB:
 
NEBuffer 1 NEBuffer 2 NEBuffer 3 NEBuffer 4
75% 100% 50% 75%
 
 Recommended buffer: 
Fermentas:Buffer G+
NEB:NEBuffer 2 + BSA 50 mM
 
 Incubation temperature:  37C
 
 Heat Inactivation:  65C for 20 minutes.
 
 Links:
 
  Mnl I, Fermentas
Mnl I, NEB
 
 



"Practical Molecular Biology" http://molbiol.edu.ru
e-mail: pmb@molbiol.edu.ru


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