> Restriction Endonucleases > Kpn I

Kpn I


 
 
 Recognition site:
 
 GGTACC
CCATGG
 
 Activity, Fermentas:
 
Blue+ Green+ Orange+ Red+ Yellow+ 1x Yellow+ 2x
20-50% 0-20% 0-20% 0-20% 20-50% 0-20%
 
 
 Activity, NEB:
 
NEBuffer 1 NEBuffer 2 NEBuffer 3 NEBuffer 4
100% 75% 0% 50%
 
 Activity, Promega:
 
 
A B C D J MULTI-CORE
100%2 2550% 2550% <10% 100% 75100%
 
 Recommended buffer: 
Fermentas:Buffer Kpn I+
NEB:NEBuffer 1 + BSA 10 mM
Promega:Buffer J
 
 Incubation temperature:  37C
 
 Heat Inactivation:  No
 
 Links:
 
  Kpn I, Fermentas
Kpn I, NEB
Promega
 
 



"Practical Molecular Biology" http://molbiol.edu.ru
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