> Restriction Endonucleases > Hpa II

Hpa II


 
 
 Recognition site:
 
 CCGG
GGCC
 
 Activity, Fermentas:
 
Blue+ Green+ Orange+ Red+ Yellow+ 1x Yellow+ 2x
50-100% 50-100% 0-20% 20-50% 100% 20-50%
 
 
 Activity, NEB:
 
NEBuffer 1 NEBuffer 2 NEBuffer 3 NEBuffer 4
100% 50% 10% 100%
 
 Activity, Promega:
 
 
A B C D MULTI-CORE
100% 5075% 5075% 1025% 100%
 
 Recommended buffer: 
Fermentas:Y+/Tango
NEB:NEBuffer 1
Promega:Buffer A
 
 Incubation temperature:  37C
 
 Heat Inactivation:  65C for 20 minutes.
 
 Links:
 
  Hpa II, Fermentas
Hpa II, NEB
Promega
 
 



"Practical Molecular Biology" http://molbiol.edu.ru
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