> Restriction Endonucleases > Hind III

Hind III


 
 
 Recognition site:
 
 AAGCTT
TTCGAA
 
 Activity, Fermentas:
 
Blue+ Green+ Orange+ Red+ Yellow+ 1x Yellow+ 2x
0-20% 20-50% 0-20% 100% 50-100% 50-100%
 
 
 Activity, NEB:
 
NEBuffer 1 NEBuffer 2 NEBuffer 3 NEBuffer 4
50% 100% 10% 50%
 
 Activity, Promega:
 
 
A B C D E MULTI-CORE
2550% 100% 75100% 1025% 100% 5075%
 
 Recommended buffer: 
Fermentas:Buffer R+
NEB:NEBuffer 2
Promega:Buffer E
 
 Incubation temperature:  37C
 
 Heat Inactivation:  65C for 20 minutes.
 
 Links:
 
  Hind III, Fermentas
Hind III, NEB
Promega
 
 



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