> Restriction Endonucleases > EcoR V

EcoR V


 
 
 Recognition site:
 
 GATATC
CTATAG
 
 
 Activity, NEB:
 
NEBuffer 1 NEBuffer 2 NEBuffer 3 NEBuffer 4
50% 75% 100% 50%
 
 Activity, Promega:
 
 
A B C D MULTI-CORE
1025% 2550% 5075% 100% 100%
 
 Recommended buffer: 
NEB:NEBuffer 3 + BSA 100 mM
Promega:Buffer D
 
 Incubation temperature:  37C
 
 Heat Inactivation:  80C for 20 minutes.
 
 Links:
 
 
EcoR V, NEB
Promega
 
 



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