> Restriction Endonucleases > EcoR I

EcoR I


 
 
 Recognition site:
 
 GAATTC
CTTAAG
 
 Activity, Fermentas:
 
Blue+ Green+ Orange+ Red+ Yellow+ 1x Yellow+ 2x
0-20% NR(20-50)% 100% 100*% NR(100)% 100%
 
 
 Activity, NEB:
 
NEBuffer 1 NEBuffer 2 NEBuffer 3 NEBuffer 4
100% 100% 100% 100%
 
 Activity, Promega:
 
 
A B C D H MULTI-CORE
2550% 5075% 5075% 5075% 100% 100%2
 
 Recommended buffer: 
Fermentas:Buffer EcoR I+
NEB:NEBuffer EcoR I
Promega:Buffer H
 
 Incubation temperature:  37C
 
 Heat Inactivation:  65C for 20 minutes.
 
 Links:
 
  EcoR I, Fermentas
EcoR I, NEB
Promega
 
 



"Practical Molecular Biology" http://molbiol.edu.ru
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