> Restriction Endonucleases > Acc65 I

Acc65 I


 
 
 Recognition site:
 
 GGTACC
CCATGG
 
 Activity, Fermentas:
 
Blue+ Green+ Orange+ Red+ Yellow+ 1x Yellow+ 2x
0-20% 20-50% 100% 20-50% 20-50% 50-100%
 
 
 Activity, NEB:
 
NEBuffer 1 NEBuffer 2 NEBuffer 3 NEBuffer 4
10% 75% 100% 25%
 
 Activity, Promega:
 
 
A B C D MULTI-CORE
1025% 5075% 75100% 100% 100%
 
 Recommended buffer: 
Fermentas:Buffer O+
NEB:NEBuffer 3 + BSA 100 mM
Promega:Buffer D
 
 Incubation temperature:  37C
 
 Heat Inactivation:  65C for 20 minutes.
 
 Links:
 
  Acc65 I, Fermentas
Acc65 I, NEB
Promega
 
 



"Practical Molecular Biology" http://molbiol.edu.ru
e-mail: pmb@molbiol.edu.ru


Rambler's Top100