> Restriction Endonucleases > Aat II

Aat II


 
 
 Recognition site:
 
 GACGTC
CTGCAG
 
 Activity, Fermentas:
 
Blue+ Green+ Orange+ Red+ Yellow+ 1x Yellow+ 2x
50-100% 20-50% 0-20% 0-20% 100% 20-50%
 
 
 Activity, NEB:
 
NEBuffer 1 NEBuffer 2 NEBuffer 3 NEBuffer 4
0% 50% 50% 100%
 
 Activity, Promega:
 
 
A B C D J MULTI-CORE
5075% 1025% <10% <10% 100% <10%
 
 Recommended buffer: 
Fermentas:Y+/Tango
NEB:NEBuffer 4
Promega:Buffer J
 
 Incubation temperature:  37C
 
 Heat Inactivation:  65C for 20 minutes.
 
 Links:
 
  Aat II, Fermentas
Aat II, NEB
Promega
 
 



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